GCaMP7 labelled neurons of the live, adult drosophila brain at 45µm depth, imaged with fast, closed-loop adaptive optics (AO) on a Light-Sheet microscope, using our new direct wavefront sensing for microscopy approach – not requiring any guide star. All this has been made possible thanks to a great collaboration with ESPCI – LPEM and Neuro-PSI, through the InovAO project (ANR-AAPG 2018 funding). More detailed science in our previous common publication here.
We recently communicated the latest advances and results about this method and other implementations of our adaptive optics technology in microscopy at Focus on Microscopy, March 28-31 2021: the 5 talks we contributed to detailed how adaptive optics can boost imaging performance in Light-Sheet and Single-Molecule Localization Microscopy.